5IMM

Nanobody targeting mouse Vsig4 in Spacegroup P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	29.598, 50.285, 163.209
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.810 - 1.200
R-factor	0.1566
Rwork	0.156
R-free	0.17550
RMSD bond length	0.009
RMSD bond angle	1.330
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.810
High resolution limit [Å]	1.200
Number of reflections	75159
<I/σ(I)>	8.06
Completeness [%]	97.0
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.2M Ammonium sulfate, 0.1M BIS-Tris, 25% PEG 3350 pH 5.5