5IM0

Crystal structure of the RNA recognition motif of mRNA decay regulator AUF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-05
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	39.068, 39.411, 93.252
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.594 - 1.700
R-factor	0.1733
Rwork	0.170
R-free	0.20400
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.987
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.700
Number of reflections	16559
<I/σ(I)>	42.2
Completeness [%]	99.9
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 3350, HEPES