5ILS
Autoinhibited ETV1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 50.186, 50.186, 69.332 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.101 - 1.399 |
| R-factor | 0.1577 |
| Rwork | 0.157 |
| R-free | 0.17830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.175 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 55.000 | 55.000 | 1.450 |
| High resolution limit [Å] | 1.399 | 3.020 | 1.400 |
| Rmerge | 0.104 | 0.063 | |
| Number of reflections | 20493 | ||
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 34.4 | 43.3 | 25.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG 5000 MME, 200 mM (NH4)2SO4, 100 mM MES |
