5IL7
Leucine rich repeat domain of the Chlorobium tepidum Roco protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.223, 98.941, 155.397 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.140 - 2.300 |
| R-factor | 0.21649 |
| Rwork | 0.214 |
| R-free | 0.26082 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 106v |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.124 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.140 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.350 |
| Number of reflections | 37250 | |
| <I/σ(I)> | 13.77 | 4.42 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Na Acetate, 2.2 M Ammonium sulfate |
