5IL6
Crystal structure of the dehydratase domain of RzxB from Pseudomonas fluorescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.480, 105.080, 68.140 |
| Unit cell angles | 90.00, 111.91, 90.00 |
Refinement procedure
| Resolution | 63.217 - 1.900 |
| R-factor | 0.2011 |
| Rwork | 0.200 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kg9 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.658 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10pre_2131: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.217 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.067 | 0.482 |
| Number of reflections | 50913 | |
| <I/σ(I)> | 13.1 | 2.4 |
| Completeness [%] | 98.1 | 97.3 |
| Redundancy | 3.3 | 3.2 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M BisTris 6.5, 0.2 M NaCl, 20% PEG6000 |
