5IJX

Crystal Structure of a C-terminally truncated Coccidioides posadasii mitochondrial tyrosyl-tRNA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	C 1 2 1
Unit cell lengths	117.173, 75.785, 52.316
Unit cell angles	90.00, 94.55, 90.00

Refinement procedure

Resolution	27.679 - 2.630
R-factor	0.2079
Rwork	0.206
R-free	0.23560
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.540
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	autoSHARP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.630
Number of reflections	12810
<I/σ(I)>	40.9
Completeness [%]	94.1
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	10 mM HEPES-NaOH pH 7.0, 8% PEG 3350, 1% isopropanol, 1 mM L-tyrosine, 12% glycerol