5IJW
Glutamate Racemase (MurI) from Mycobacterium smegmatis with bound D-glutamate, 1.8 Angstrom resolution, X-ray diffraction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-03-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.100, 90.100, 101.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.400 - 1.760 |
| Rwork | 0.170 |
| R-free | 0.20530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gzm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.848 |
| Data reduction software | CrystalClear (2.0) |
| Data scaling software | CrystalClear (2.0) |
| Phasing software | CrystalClear (2.0) |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.400 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.109 | 0.325 |
| Number of reflections | 54647 | |
| <I/σ(I)> | 11 | 4.5 |
| Completeness [%] | 97.0 | 90.2 |
| Redundancy | 6.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 18% PEG 3350, 0.2 M NaI |
