5IJP
Crystal structure of the SPX domain of Chaetomium thermophilum Vtc4 in complex with inositol hexakisphosphate (InsP6).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-02 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 62 |
| Unit cell lengths | 74.200, 74.200, 74.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.630 - 2.750 |
| R-factor | 0.2429 |
| Rwork | 0.243 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iig |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.840 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.820 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 6115 | |
| <I/σ(I)> | 43 | 2.55 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 82.4 | 86 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 298 | 13% PEG 3350, 6% Tacsimate |
