5IJL
D-family DNA polymerase - DP2 subunit (catalytic subunit)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9840 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.820, 106.200, 110.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.800 - 2.190 |
| R-factor | 0.2008 |
| Rwork | 0.199 |
| R-free | 0.23460 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.800 |
| High resolution limit [Å] | 2.190 |
| Number of reflections | 63302 |
| <I/σ(I)> | 8.5 |
| Completeness [%] | 99.6 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 60mM MES pH 5.6, 300mM NaCl, 6% PEG6000 |
