5IJI
Fragment of nitrate/nitrite sensor histidine kinase NarQ (WT) in symmetric holo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.972 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 57.702, 73.830, 235.813 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.400 - 1.940 |
| R-factor | 0.19277 |
| Rwork | 0.190 |
| R-free | 0.25416 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.084 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 1.990 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.083 | 0.989 |
| Number of reflections | 17728 | |
| <I/σ(I)> | 14.5 | 2 |
| Completeness [%] | 98.9 | 98.1 |
| Redundancy | 5.5 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | in meso lipidic cubic phase crystallization |
