5IJ1
SrpA adhesin in complex with sialyllactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2015-12-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.522, 46.769, 64.761 |
| Unit cell angles | 90.00, 102.72, 90.00 |
Refinement procedure
| Resolution | 42.559 - 1.800 |
| R-factor | 0.1767 |
| Rwork | 0.176 |
| R-free | 0.19820 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
| Rmerge | 0.058 | 0.025 | 0.475 |
| Rmeas | 0.066 | ||
| Rpim | 0.032 | ||
| Total number of observations | 188088 | ||
| Number of reflections | 45316 | ||
| <I/σ(I)> | 14 | ||
| Completeness [%] | 94.4 | 98.9 | 64 |
| Redundancy | 4.2 | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | calcium acetate, sodium cacodylate, PEG 8000 |
