5IIB
Crystal structure of red abalone egg VERL repeat 3 in complex with sperm lysin at 1.64 A resolution (crystal form II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.500, 64.500, 129.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.250 - 1.640 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2lis |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.908 |
| Data reduction software | XDS (20141118) |
| Data scaling software | XDS (20141118) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.250 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.048 | 2.087 |
| Number of reflections | 39159 | |
| <I/σ(I)> | 12.43 | 0.85 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.6 M ammonium sulfate, 0.1 M citric acid |
