5II4
Crystal structure of red abalone VERL repeat 1 with linker at 2.0 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 107.120, 107.120, 195.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.991 - 2.000 |
| R-factor | 0.2193 |
| Rwork | 0.218 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3set 3SEX and 4WRN |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.907 |
| Data reduction software | XDS (20141103) |
| Data scaling software | XDS (20141103) |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev-1894_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.991 | 2.072 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.103 | 1.446 |
| Number of reflections | 44601 | |
| <I/σ(I)> | 8.84 | 1.01 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 4.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 40% PEG 600, 0.1M CHES |
