5II0
Crystal structure of the human calcitonin receptor ectodomain in complex with a truncated salmon calcitonin analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002+ |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54179 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.558, 113.165, 55.424 |
Unit cell angles | 90.00, 114.79, 90.00 |
Refinement procedure
Resolution | 24.840 - 2.097 |
R-factor | 0.1721 |
Rwork | 0.170 |
R-free | 0.20550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3n7s |
RMSD bond length | 0.008 |
RMSD bond angle | 1.097 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1938) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.840 | 2.172 |
High resolution limit [Å] | 2.097 | 2.097 |
Rmerge | 0.081 | 0.544 |
Number of reflections | 31627 | |
<I/σ(I)> | 15.99 | 2.86 |
Completeness [%] | 97.0 | 100 |
Redundancy | 7.5 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1 M Hepes, pH 7.5, 4.3 M sodium chloride, 2 M urea |