5II0
Crystal structure of the human calcitonin receptor ectodomain in complex with a truncated salmon calcitonin analogue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002+ |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54179 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.558, 113.165, 55.424 |
| Unit cell angles | 90.00, 114.79, 90.00 |
Refinement procedure
| Resolution | 24.840 - 2.097 |
| R-factor | 0.1721 |
| Rwork | 0.170 |
| R-free | 0.20550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n7s |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.097 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1938) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.840 | 2.172 |
| High resolution limit [Å] | 2.097 | 2.097 |
| Rmerge | 0.081 | 0.544 |
| Number of reflections | 31627 | |
| <I/σ(I)> | 15.99 | 2.86 |
| Completeness [%] | 97.0 | 100 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1 M Hepes, pH 7.5, 4.3 M sodium chloride, 2 M urea |
