5IHZ
Crystal structure of anti-gliadin 1002-1E01 Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.270, 147.810, 95.230 |
Unit cell angles | 90.00, 103.50, 90.00 |
Refinement procedure
Resolution | 78.472 - 1.640 |
R-factor | 0.2018 |
Rwork | 0.200 |
R-free | 0.22850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4q9q |
RMSD bond length | 0.010 |
RMSD bond angle | 1.272 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.600 | 1.680 |
High resolution limit [Å] | 1.640 | 1.640 |
Number of reflections | 240238 | |
<I/σ(I)> | 18.3 | |
Completeness [%] | 98.8 | |
Redundancy | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20 %w/v Polyethylene Glycol 3350, 0.200 M Potassium Sulphate |