5IHY

The crystal structure of Bacillus subtilis SeMet-YpgQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-24
Detector	ADSC Quantum Q270r
Wavelength(s)	0.97937
Spacegroup name	P 1 21 1
Unit cell lengths	47.487, 53.087, 83.388
Unit cell angles	90.00, 93.70, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.21734
Rwork	0.215
R-free	0.26145
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.999
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	14383
<I/σ(I)>	29.4
Completeness [%]	99.1
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	40% PEG 600, 0.1 M sodium citrate