5IH6

Human Casein Kinase 1 isoform delta (kinase domain) in complex with Epiblastin A derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.76995
Spacegroup name	P 31 2 1
Unit cell lengths	65.720, 65.720, 151.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.800 - 2.300
R-factor	0.2344
Rwork	0.233
R-free	0.25990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih4
RMSD bond length	0.013
RMSD bond angle	1.552
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.800		2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.115	0.047	1.481
Rmeas	0.119	0.049	1.597
Total number of observations	243801
Number of reflections	17269	239	1131
<I/σ(I)>	12.49	42.96	1.07
Completeness [%]	98.3	97.6	88.8
Redundancy	14.1		7.1
CC(1/2)	0.999	0.999	0.232

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M Li2SO4, 0.7 - 0.8 M Na-K tartrate, 0.1 M CHES