5IH4

Human Casein Kinase 1 isoform delta apo (kinase domain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.916
Spacegroup name	P 31 2 1
Unit cell lengths	64.999, 64.999, 152.881
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.000 - 1.900
R-factor	0.1927
Rwork	0.191
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uys
RMSD bond length	0.020
RMSD bond angle	2.061
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.000		1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.116	0.035	0.869
Number of reflections	29688
<I/σ(I)>	19.16	55.04	4.14
Completeness [%]	97.9	98.5	81.3
Redundancy	16.7
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	277.15	0.1 M Li2SO4, 0.7 - 0.8 M Na-K tartrate, 0.1 M CHES (pH 9.5)