5IH2
Structure, thermodynamics, and the role of conformational dynamics in the interactions between the N-terminal SH3 domain of CrkII and proline-rich motifs in cAbl
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-02-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.222, 29.479, 45.756 |
| Unit cell angles | 90.00, 94.31, 90.00 |
Refinement procedure
| Resolution | 45.630 - 1.800 |
| R-factor | 0.17323 |
| Rwork | 0.170 |
| R-free | 0.24252 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cka |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.039 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.144 | |
| Number of reflections | 11478 | |
| <I/σ(I)> | 38.055 | |
| Completeness [%] | 99.1 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 |
