5IFW
Quantitative interaction mapping reveals an extended ubiquitin regulatory domain in ASPL that disrupts functional p97 hexamers and induces cell death
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 143.344, 208.361, 99.129 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.887 - 3.400 |
| R-factor | 0.2307 |
| Rwork | 0.229 |
| R-free | 0.26140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cf1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.864 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.900 | 3.490 | |
| High resolution limit [Å] | 3.400 | 15.210 | 3.400 |
| Rmerge | 0.168 | 0.025 | 0.844 |
| Number of reflections | 20794 | ||
| <I/σ(I)> | 10.8 | 39.22 | 2.16 |
| Completeness [%] | 99.8 | 86.4 | 100 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 2M Ammonium sulfate, 0.1M Bis-Tris pH 5.5 |
