5IFR
Structure of the stable UBE2D3-UbDha conjugate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-09 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.125, 52.271, 53.624 |
| Unit cell angles | 90.00, 100.78, 90.00 |
Refinement procedure
| Resolution | 52.678 - 2.200 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ugb |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.580 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.680 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.077 | 0.760 |
| Number of reflections | 11594 | |
| <I/σ(I)> | 8.8 | 2 |
| Completeness [%] | 98.6 | 99.6 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 200mM tripotassium citrate, 20% PEG 3350 |
