5IFO
X-ray structure of HSA-Myr-KP1019
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.973 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.110, 38.060, 94.950 |
| Unit cell angles | 90.00, 105.06, 90.00 |
Refinement procedure
| Resolution | 45.844 - 3.200 |
| R-factor | 0.245 |
| Rwork | 0.244 |
| R-free | 0.26150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b9m |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.413 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX ((1.10_2155)) |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.844 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 10508 |
| <I/σ(I)> | 17.89 |
| Completeness [%] | 98.0 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 75-120 mg/ml protein, 50 mM potassium phosphate, 25-30% PEG4000, 150 mM KCl, 5 mM sodium azide |
