5IFE

Crystal structure of the human SF3b core complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.918, 1.254, 1.282, 1.0
Spacegroup name	P 21 21 21
Unit cell lengths	105.055, 154.436, 210.418
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.037 - 3.100
R-factor	0.232
Rwork	0.231
R-free	0.25950
Structure solution method	SIRAS
RMSD bond length	0.002
RMSD bond angle	0.539
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	autoSHARP
Refinement software	PHENIX (1.10.1-2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.040
High resolution limit [Å]	3.100
Number of reflections	62814
<I/σ(I)>	10.5
Completeness [%]	99.9
Redundancy	8.3
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.05 M HEPES-NaOH pH=6.91-7.05, 40% Pentaerythritol propoxylate (5/4 PO/OH), 0.2M KCl