5IFA

Crystal structure of unbound VRC01c-HuGL2 Fab from an HIV-1 naive donor at 1.82 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	293
Detector technology	PIXEL
Collection date	2015-04-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 2
Unit cell lengths	79.063, 124.201, 47.946
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.837 - 1.821
R-factor	0.2065
Rwork	0.205
R-free	0.24030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jpk
RMSD bond length	0.009
RMSD bond angle	1.038
Data reduction software	HKL-2000 (v706e)
Data scaling software	HKL-2000 (v706e)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.840
High resolution limit [Å]	1.820
Number of reflections	40894
<I/σ(I)>	0.95
Completeness [%]	97.8
Redundancy	4.7
CC(1/2)	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M lithium sulfate and 20% (w/v) PEG3350, and cryo-protected with 25% glycerol