5IEH
Structure of HLA-B*40:02 in complex with the phosphorylated endogenous peptide REF(p)SKEPEL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00556 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.044, 82.249, 110.084 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.863 - 1.500 |
| R-factor | 0.1619 |
| Rwork | 0.161 |
| R-free | 0.18330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.012 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10) |
Data quality characteristics
| Overall | |
| Number of reflections | 128337 |
| <I/σ(I)> | 22.9 |
| Completeness [%] | 99.9 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 150 mM Tris 8.0, 20% PEG5K, 8% ethylene glycol |
