5IEG
Murine endoplasmic reticulum alpha-glucosidase II with N-9'-methoxynonyl-1-deoxynojirimycin.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.07205 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 104.321, 173.311, 63.032 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 89.380 - 1.822 |
R-factor | 0.176 |
Rwork | 0.175 |
R-free | 0.19500 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5f0e |
RMSD bond length | 0.010 |
RMSD bond angle | 1.010 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | BUSTER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 104.321 | 1.828 |
High resolution limit [Å] | 1.822 | 1.822 |
Number of reflections | 102902 | |
<I/σ(I)> | 12.1 | 2.1 |
Completeness [%] | 100.0 | 97.5 |
Redundancy | 6.7 | 6.9 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 21% v/v ethylene glycol, 11% w/v PEG 8000 (from the Morpheus Precipitant Mix 2), 50 mM Morpheus carboxylic acids mix, 100 mM Morpheus buffer system 1 pH 6.25 |