5IED
Murine endoplasmic reticulum alpha-glucosidase II with castanospermine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 103.430, 173.900, 63.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.950 - 1.810 |
| R-factor | 0.16 |
| Rwork | 0.159 |
| R-free | 0.18400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5f0e |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 173.900 | 1.910 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Number of reflections | 103854 | |
| <I/σ(I)> | 8.8 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.6 |
| CC(1/2) | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 21% v/v ethylene glycol, 11% w/v PEG 8000 (from the Morpheus Precipitant Mix 2), 50 mM Morpheus carboxylic acids mix, 100 mM Morpheus buffer system 1 pH 6.25 |
