5IDL

Crystal structure of the germline-targeting HIV-1 gp120 engineered outer domain, eOD-GT8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	P 21 21 21
Unit cell lengths	34.330, 53.060, 77.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.422 - 2.900
R-factor	0.2575
Rwork	0.253
R-free	0.29710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jpj
RMSD bond length	0.002
RMSD bond angle	0.455
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000		2.920
High resolution limit [Å]	2.840	12.710	2.840
Rmerge	0.131	0.033	0.779
Number of reflections	3333
<I/σ(I)>	6.93	24.59	1.4
Completeness [%]	99.4	95.7	98.9
Redundancy	5.8
CC(1/2)	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	2% (v/v) PEG 400, 2M ammonium sulfate, 0.1M HEPES pH 7.5