5IDJ
Bifunctional histidine kinase CckA (domains DHp-CA) in complex with ADP/Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 164.856, 164.856, 48.001 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.590 - 3.020 |
| R-factor | 0.2416 |
| Rwork | 0.239 |
| R-free | 0.28860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5idm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.645 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 142.598 | 3.170 |
| High resolution limit [Å] | 3.010 | 3.010 |
| Rmerge | 0.327 | |
| Number of reflections | 7413 | 7413 |
| <I/σ(I)> | 15 | 2.2 |
| Completeness [%] | 91.4 | 51.2 |
| Redundancy | 11.3 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 30% PEG 550 MME, 30% PEG 2000 MME, Morpheus buffer 1 pH 6.5 and Morpheus carboxylic acids |
