5IDA
Crystal structure of CGL1 from Crassostrea gigas, mannose-bound form (CGL1/MAN)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-11-01 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.723, 58.778, 108.460 |
| Unit cell angles | 90.00, 93.72, 90.00 |
Refinement procedure
| Resolution | 17.870 - 1.100 |
| R-factor | 0.16705 |
| Rwork | 0.164 |
| R-free | 0.22207 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.447 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.100 |
| Number of reflections | 160510 |
| <I/σ(I)> | 17 |
| Completeness [%] | 79.6 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 0.2 M magnesium acetate, 0.1 M sodium cacodylate, 20% PEG8000 |
