5IBV

Crystal Structure of Human Astrovirus capsid protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Detector technology	PIXEL
Collection date	2014-11-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	51.983, 59.236, 56.197
Unit cell angles	90.00, 91.45, 90.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.2132
Rwork	0.209
R-free	0.24580
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.207
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.066	0.507
Number of reflections	18278
<I/σ(I)>	17.9	2
Completeness [%]	99.8	97.8
Redundancy	4	3.2
CC(1/2)	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	VAPOR DIFFUSION, HANGING DROP, 0.2 M ammoium phosphate, 22% PEG 4000