5IBL
Human antibody 6639 in complex with influenza hemagglutinin H1 X-181
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 173.15 |
| Detector technology | CCD |
| Collection date | 2014-08-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979200 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.130, 104.720, 116.660 |
| Unit cell angles | 101.95, 95.33, 98.36 |
Refinement procedure
| Resolution | 44.810 - 3.390 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ibu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.810 | 3.590 |
| High resolution limit [Å] | 3.390 | 3.390 |
| Rmerge | 0.178 | 1.730 |
| Number of reflections | 32602 | |
| <I/σ(I)> | 4.3 | 0.48 |
| Completeness [%] | 95.5 | 94 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 12% (w/v) PEG 8000, 10% (w/v) MPD and 25 mM KH2PO4 |
