5IBK

Skp1-F-box in complex with a ubiquitin variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	63.368, 98.015, 107.704
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.709 - 2.503
R-factor	0.2016
Rwork	0.200
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ovr 1ubq
RMSD bond length	0.004
RMSD bond angle	0.879
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.709	50.000	2.560
High resolution limit [Å]	2.500	6.160	2.500
Rmerge	0.046	0.021	0.657
Rmeas	0.052
Rpim	0.024
Total number of observations	103319
Number of reflections	23666
<I/σ(I)>	5.6
Completeness [%]	99.4	98.8	99.1
Redundancy	4.4	4.2	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	100 mM Acetate pH 4.5, 12% PEG 4000, 15% glyerol