5IB2
Crystal structure of HLA-B*27:05 complexed with the self-peptide pVIPR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-26 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9184 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.186, 82.073, 66.086 |
Unit cell angles | 90.00, 109.46, 90.00 |
Refinement procedure
Resolution | 29.300 - 1.440 |
R-factor | 0.1378 |
Rwork | 0.137 |
R-free | 0.16050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1of2 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.011 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: 000)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.440 | 1.440 |
Number of reflections | 92249 | |
<I/σ(I)> | 27.51 | 5.45 |
Completeness [%] | 98.8 | 97.3 |
Redundancy | 6.9 | 6.4 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 |