5IB2

Crystal structure of HLA-B*27:05 complexed with the self-peptide pVIPR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-26
Detector	RAYONIX MX-225
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	51.186, 82.073, 66.086
Unit cell angles	90.00, 109.46, 90.00

Refinement procedure

Resolution	29.300 - 1.440
R-factor	0.1378
Rwork	0.137
R-free	0.16050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1of2
RMSD bond length	0.008
RMSD bond angle	1.011
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.440	1.440
Number of reflections	92249
<I/σ(I)>	27.51	5.45
Completeness [%]	98.8	97.3
Redundancy	6.9	6.4
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291