5I9J

Structure of the cholesterol and lutein-binding domain of human STARD3 at 1.74A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11583
Spacegroup name	P 31 2 1
Unit cell lengths	83.390, 83.390, 82.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.180 - 1.740
R-factor	0.17
Rwork	0.169
R-free	0.19190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1em2
RMSD bond length	0.013
RMSD bond angle	1.161
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev-2276)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.180	1.790
High resolution limit [Å]	1.740	1.740
Rmerge	0.046	0.810
Number of reflections	34160
<I/σ(I)>	25.8	2.4
Completeness [%]	99.5	98.6
Redundancy	8.8	6.8
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.1	295	0.85 M sodium/potassium tartrate, 0.2 M lithium sulfate, 0.1 M CHES, 0.01 M dithiothreitol. Cryostabilizer: 18% ethylene glycol, 5% ethanol in addition to 0.8M sodium/potassium tartrate, 0.1 M CHES pH 9.1, 0.2 M lithium sulfate, 0.01 M dithiothreitol. Crystals were frozen in propane and stored under liquid nitrogen