5I9H
Crystal structure of designed pentatricopeptide repeat protein dPPR-U8G2 in complex with its target RNA U8G2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9784 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.063, 85.098, 95.397 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.699 - 2.504 |
R-factor | 0.2268 |
Rwork | 0.223 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pjr |
RMSD bond length | 0.007 |
RMSD bond angle | 0.917 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 15179 |
<I/σ(I)> | 19.8 |
Completeness [%] | 99.8 |
Redundancy | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 3350, Bis-Tris propane, Magnesium chloride, Ethyl Acetate, D-(+)-glucose monohydrate |