5I9E

Crystal structure of a nuclear actin ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	110.260, 202.220, 87.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.403 - 2.800
R-factor	0.2338
Rwork	0.232
R-free	0.28440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qb0 1yag
RMSD bond length	0.007
RMSD bond angle	0.708
Data reduction software	HKL-2000 (V705)
Data scaling software	HKL-2000 (V705)
Phasing software	PHASER (5)
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.403	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.120
Number of reflections	48531
<I/σ(I)>	15.4
Completeness [%]	99.7
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	polyethylene glycol (PEG) 3350, citric acid, BIS-TRIS propane