5I92

Crystal structure of Glutamate-1-semialdehyde 2,1- aminomutase (GSA) from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	70.660, 121.440, 142.740
Unit cell angles	90.00, 93.27, 90.00

Refinement procedure

Resolution	47.503 - 1.750
R-factor	0.153
Rwork	0.153
R-free	0.17880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.880
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	BALBES
Refinement software	PHENIX ((dev_2313))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.520		1.800
High resolution limit [Å]	1.750	7.830	1.750
Rmerge	0.056	0.021	0.482
Rmeas	0.064	0.024	0.550
Total number of observations	1022137
Number of reflections	241258	2744	17795
<I/σ(I)>	18.2	50.11	3.33
Completeness [%]	99.9	98.2	99.9
CC(1/2)	0.999	0.999	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	MCSG-1 (D9): 25% PEG-3350, 0.2M NACL, 0.1M TRIS, PH=8.5, CRYO: 20% EG