5I90

Crystal Structure of PvdN from Pseudomonas Aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-15
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	62.521, 98.996, 63.046
Unit cell angles	90.00, 102.32, 90.00

Refinement procedure

Resolution	29.407 - 1.219
R-factor	0.1405
Rwork	0.140
R-free	0.15830
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.875
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.407	1.263
High resolution limit [Å]	1.219	1.219
Number of reflections	221938
<I/σ(I)>	22.92
Completeness [%]	99.9	98.99
Redundancy	4.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	287	10-14% PEG 8000, 100-200 mM Glycine, 50 mM MES (pH 6.0)