5I8J

Crystal structure of Dmd from phage RB69

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 1W2B
Synchrotron site	BSRF
Beamline	1W2B
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-05
Detector	MAR CCD 165 mm
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	31.910, 53.933, 75.454
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.914 - 1.750
R-factor	0.1934
Rwork	0.192
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4I8R
RMSD bond length	0.006
RMSD bond angle	0.964
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.084	0.333
Number of reflections	13857
<I/σ(I)>	53.76	8.81
Completeness [%]	99.9	99.3
Redundancy	13.7	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M HEPES 25% PEG3350