5I7P

Crystal structure of Fkbp12-IF(SlyD), a chimeric protein of human Fkbp12 and the insert in flap domain of Ecoli SlyD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-06-17
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	60.219, 60.219, 120.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.375 - 2.002
R-factor	0.2208
Rwork	0.219
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fkk
RMSD bond length	0.003
RMSD bond angle	0.538
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10pre_2131: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.375	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.061	0.560
Number of reflections	15492
<I/σ(I)>	22	2.6
Completeness [%]	99.2	99.2
Redundancy	5.9	5.8
CC(1/2)	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20 % PEG3350, 0.1 M MgCl2, 0.1 M Hepes pH 7.5