5I79
Crystal structure of a beta-1,4-endoglucanase mutant from Aspergillus niger in complex with sugar
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97622 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.992, 51.332, 75.358 |
| Unit cell angles | 90.00, 95.20, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.350 |
| R-factor | 0.15 |
| Rwork | 0.147 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gzj |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.170 | 0.318 |
| Number of reflections | 25929 | |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 99.1 | 96.2 |
| Redundancy | 4.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG600, Ca(OAC)2, cacodylate |
