5I72

Crystal structure of the oligomeric form of the Lassa virus matrix protein Z

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-10
Detector	3 x 3 CCD array (ADSC Q315R)
Wavelength(s)	1.2827
Spacegroup name	H 3 2
Unit cell lengths	116.961, 116.961, 82.544
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.221 - 2.900
R-factor	0.1913
Rwork	0.190
R-free	0.20930
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.371
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.221	38.210	3.000
High resolution limit [Å]	2.900	6.240	2.900
Rmerge	0.074	0.052	0.390
Rmeas	0.083	0.059	0.440
Total number of observations	47129	4684	4846
Number of reflections	9325
<I/σ(I)>	10.2	21.3	2.8
Completeness [%]	99.8	99.3	100
Redundancy	4.8	4.68	4.73

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	300-400mM ammonium sulfate, 100mM HEPES pH 7.5 and 17% PEG 3350