5I72
Crystal structure of the oligomeric form of the Lassa virus matrix protein Z
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-10 |
| Detector | 3 x 3 CCD array (ADSC Q315R) |
| Wavelength(s) | 1.2827 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 116.961, 116.961, 82.544 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.221 - 2.900 |
| R-factor | 0.1913 |
| Rwork | 0.190 |
| R-free | 0.20930 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.371 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.221 | 38.210 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
| Rmerge | 0.074 | 0.052 | 0.390 |
| Rmeas | 0.083 | 0.059 | 0.440 |
| Total number of observations | 47129 | 4684 | 4846 |
| Number of reflections | 9325 | ||
| <I/σ(I)> | 10.2 | 21.3 | 2.8 |
| Completeness [%] | 99.8 | 99.3 | 100 |
| Redundancy | 4.8 | 4.68 | 4.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 300-400mM ammonium sulfate, 100mM HEPES pH 7.5 and 17% PEG 3350 |
