5I6Q
Crystal structure of color device state B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-08-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.643, 69.012, 69.265 |
| Unit cell angles | 99.02, 96.63, 100.28 |
Refinement procedure
| Resolution | 33.389 - 4.912 |
| R-factor | 0.1555 |
| Rwork | 0.148 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gbi |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.262 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2152)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 5.470 |
| High resolution limit [Å] | 4.912 | 14.490 | 4.912 |
| Rmerge | 0.068 | 0.051 | 0.539 |
| Total number of observations | 14123 | ||
| Number of reflections | 4712 | ||
| <I/σ(I)> | 10.1 | ||
| Completeness [%] | 97.0 | 87.5 | 84 |
| Redundancy | 3.5 | 3.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 30 mM sodium cacodylate, 50 mM magnesium acetate, 50 mM ammonium sulfate, 5 mM magnesium chloride and 25 mM Tris?HCl (pH 8.5) |
