5I6I
Crystal structure of a dBCCP-variant of Chaetomium thermophilum acetyl-CoA carboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99986 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 462.200, 462.200, 204.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.950 - 8.400 |
| R-factor | 0.2981 |
| Rwork | 0.297 |
| R-free | 0.32360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i6f 5i6e |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS (VERSION March 1, 2015) |
| Data scaling software | XSCALE (VERSION March 1, 2015) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.950 | 8.620 |
| High resolution limit [Å] | 8.400 | 8.400 |
| Rmerge | 0.294 | 3.820 |
| Number of reflections | 12113 | |
| <I/σ(I)> | 9.05 | 0.9 |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 18.5 | 18.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292.15 | Morpheus buffer system 3, Morpheus ethylene glycol mix, PEG4000, Glycerol |
