5I6H
Crystal structure of CD-CT domains of Chaetomium thermophilum acetyl-CoA carboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-20 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 295.020, 295.020, 189.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.850 - 7.200 |
R-factor | 0.233 |
Rwork | 0.232 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5i6f 5i6e |
RMSD bond length | 0.010 |
RMSD bond angle | 1.150 |
Data reduction software | XDS (VERSION March 1, 2015) |
Data scaling software | XSCALE (VERSION March 1, 2015) |
Phasing software | PHASER (2.5.7) |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.850 | 7.390 |
High resolution limit [Å] | 7.200 | 7.200 |
Rmerge | 0.056 | 3.030 |
Number of reflections | 14050 | |
<I/σ(I)> | 18.95 | 0.92 |
Completeness [%] | 99.6 | 100 |
Redundancy | 9.9 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292.15 | Bicine, PEG8000 |