5I66
X-ray structure of the Fab fragment of 8B6, a murine monoclonal antibody specific for the human serotonin transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 81.560, 81.560, 142.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.672 - 1.624 |
R-factor | 0.1792 |
Rwork | 0.178 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lqf |
RMSD bond length | 0.012 |
RMSD bond angle | 1.148 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2142: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 57.672 |
High resolution limit [Å] | 1.620 |
Rmerge | 0.052 |
Number of reflections | 117803 |
<I/σ(I)> | 18.9 |
Completeness [%] | 97.7 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM HEPES pH 7.5, 300 mM NH4H2PO4 and 22.5% pure PEG (0.3-0.8 kDa) |