5I5K
Structure of complement C5 in complex with eculizumab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 197.704, 269.321, 202.824 |
| Unit cell angles | 90.00, 98.58, 90.00 |
Refinement procedure
| Resolution | 49.795 - 4.200 |
| R-factor | 0.2055 |
| Rwork | 0.204 |
| R-free | 0.24360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kmt 3pvm |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.641 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.800 | |
| High resolution limit [Å] | 4.200 | 4.200 |
| Number of reflections | 76073 | |
| <I/σ(I)> | 6.28 | 1.29 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 4.9 | 5.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1 M Imidazole pH 6.2 4% v/v Tacsimate pH 7 8% PEG 3350 |
