5I54

Exploring onset of lysozyme denaturation by urea - soak period 4 hours

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-08-01
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	79.040, 79.040, 36.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.945 - 1.608
R-factor	0.1663
Rwork	0.165
R-free	0.19930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.006
RMSD bond angle	1.028
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	27.950
High resolution limit [Å]	1.608
Rmerge	0.020
Number of reflections	15566
<I/σ(I)>	30.22
Completeness [%]	99.8
Redundancy	2
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	295	Sodium chloride, sodium citrate