5I4X

Exploring onset of lysozyme denaturation by urea - soak period 2 hours

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-10-20
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	78.840, 78.840, 36.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.730 - 1.607
R-factor	0.1793
Rwork	0.178
R-free	0.21270
RMSD bond length	0.006
RMSD bond angle	1.014
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.730
High resolution limit [Å]	1.607
Rmerge	0.036
Number of reflections	15398
<I/σ(I)>	14.29
Completeness [%]	98.5
Redundancy	1.9
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	295	Sodium chloride, sodium citrate